#Andy osmac mac os#
#Andy osmac software#
#Andy osmac windows#

Macintosh System Disk (690-5003A), 3.5-inch floppy containing System 1.0 (0.97) / Finder 1.0 Bruce Horn and Steve Capps wrote the Macintosh Finder, as well as a number of Macintosh system utilities.

#Andy osmac windows#

The icons of the operating system, which represent folders and application software, were designed by Susan Kare, who later designed the icons for Microsoft Windows 3.0. Additional to the system kernel is the Finder, an application used for file management, which also displays the Desktop.

#Andy osmac mac os#

As part of an agreement allowing Xerox to buy shares in Apple at a favorable price, it also used concepts from the Xerox PARC Xerox Alto, which Steve Jobs and several other Macintosh team members had previewed. The first version of Mac OS (simply called System) is easily distinguished between many other operating systems from the same period because it does not use a command line interface it was one of the first operating systems to use an entirely graphical user interface or GUI.

#Andy osmac software#

doi: 10.1111/ facts : The original Macintosh system software was partially based on the Lisa OS, previously released by Apple for the Lisa computer in 1983. antiSMASH 5.0: Updates to the secondary metabolite genome mining pipeline. doi: 10.1093/nar/gkr466.īlin K., Shaw S., Steinke K., Villebro R., Ziemert N., Lee S.Y., Medema M.H., Weber T. antiSMASH: Rapid identification, annotation and analysis of secondary metabolite biosynthesis gene clusters in bacterial and fungal genome sequences. Medema M.H., Blin K., Cimermancic P., de Jager V., Zakrzewski P., Fischbach M.A., Weber T., Takano E., Breitling R. The Sound of Silence: Activating Silent Biosynthetic Gene Clusters in Marine Microorganisms. Reen F.J., Romano S., Dobson A.D.W., O’Gara F. Identification of Secondary Metabolite Gene Clusters in the Pseudovibrio Genus Reveals Encouraging Biosynthetic Potential toward the Production of Novel Bioactive Compounds. Naughton L.M., Romano S., O’Gara F., Dobson A.D.W. Differences between groups were determined by analysis of variance (ANOVA) and Dunnett’s test was used for multiple comparisons with the control. Statistical significances are referred to the negative control.

Data are means of three independent experiments. DMSO was used as an internal negative control. Untreated cells were used as negative control (CTRL–).

Greco extract and Pleconaril were used as positive controls (CTRL+). ( e) Green Fluorescent Protein herpes simplex virus type 1 (GFP HSV-1) was used to confirm the antiviral activity of F90 at 7.8 µg/mL (absence of fluorescence as CTRL+), while around 30% of inhibition is displayed at 0.4 µg/mL (presence of fluorescence). F90 was tested, ranging from 125 to 0.4 µg/mL. The antiviral activity of F90 was evaluated against three enveloped viruses: herpes simplex virus type 1 (HSV-1) ( a), human coronavirus 229E (HCoV-229E) ( b), human coronavirus OC43 (HCoV-OC43) ( c), and the envelope-free Poliovirus PV-1 ( d). OSMAC approach antiproliferative antiviral biosurfactant glycolipids marine bacteria mass spectrometry metabolomics microbial natural products trehalolipids.Īntiviral activity of fraction 90% MeOH (F90). The identified compounds belong to the biosurfactants class, amphiphilic molecules, which play a crucial role in the biotech and biomedical industry. The active fraction showed a potent antiviral effect against enveloped viruses, such as herpes simplex virus and human coronaviruses, and high antiproliferative activity in PC3 prostate cancer cell line. This strategy led to identifying a bioactive fraction containing >30 new glycolipids holding unusual functional groups.

Notably, only a single growth condition induced the production of unique compounds, which were partially purified and structurally characterized by liquid chromatography high-resolution tandem mass spectrometry (LC-HRMS/MS). The strain was cultivated in 22 different growth media and the generated extracts were subjected to metabolomic analysis and functional screening. I2R was sequenced and analyzed by antiSMASH for the identification of biosynthetic gene clusters. In this work, the whole genome of the marine isolate Rhodococcus sp.

The untargeted approach, One Strain Many Compounds (OSMAC), is one of the most promising strategies to induce the expression of silent genes, especially when combined with genome mining and advanced metabolomics analysis. However, the redundancy of molecules in the discovery process represents a serious issue. Natural products of microbial origin have inspired most of the commercial pharmaceuticals, especially those from Actinobacteria.